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Ships within 48 hours · Estimated delivery Jul 17 - Jul 22
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Description
Human AS-IgG (anti sperm-Immunoglobulin G) ELISA KitThe Human AS IgG (anti sperm Immunoglobulin G) ELISA Kit measures AS IgG in Human Serum, Plasma. This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP detection antibody, then it binds with AS
The Human AS-IgG (anti sperm-Immunoglobulin G) ELISA Kit measures AS-IgG in Human Serum, Plasma. This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP-antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with AS-IgG bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of AS-IgG in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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Exchange/Return Notes
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