SKU: 6427373474

Porcine PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA Kit

Sale price$202.50 Regular price$225.00
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Description

Porcine PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA KitThe Porcine PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Porcine PDK4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine PDK4 and Avidin Horseradish Peroxidase (HRP) conjugate are added

The Porcine PDK4 (Pyruvate Dehydrogenase Kinase Isozyme 4) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine PDK4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine PDK4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine PDK4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine PDK4. You can calculate the concentration of Porcine PDK4 in the samples by comparing the OD of the samples to the standard curve.

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SKU: 6427373474

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4.7 ★★★★★
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MTmarie
Lexington, US
★★★★★ 5
Great choice for heavy chewers
Perfect for dogs that chew hard! Ours shred comparable toys at every turn, but this has held up beautifully. We’ve had it for a few months and it’s got minor wear and tear, but it is easy for them to play with, hardy, and worth every penny.
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Reviewed in the United States on April 1, 2026
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Sharon
Lexington, US
★★★★★ 5
Durable, Fun, soft enough to not break teeth
Finally found a quality toy that our 75lb Black Mouth Cur hasn't destroyed in 5 minutes! In fact it's been a few months now. There are teeth marks, but she hasn't been able to take a chunk out of it yet. It even has some bounce to it when you toss it. And she finds it fun. Veterinary dentists recommend toys that are soft enough that you can leave a fingernail print in for toys that are less likely to break teeth, unlike bones and antlers. This is that toy! (My other dog broke both of her upper 3 rooted premolars on a Nylabone - healthy roots but the slab fracture exposed the pulp and had to be removed. Healthy roots are more difficult to remove. Dentists say those and antlers are money makers. 😭)
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Reviewed in the United States on February 17, 2026
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Verified Purchase
KBruner
Carnegie, US
★★★★★ 5
Great toy for big chewers!
Have only had 3 days but my boy has been trying to destroy it and it’s holding up really well! Normally his toys would have been destroyed in 24 hours or less, including Kong and other brands advertised as tough!
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Reviewed in the United States on May 13, 2026
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Verified Purchase
P. Purcell
San Leandro, US
★★★★★ 5
Durability is no joke!
Excellent product! Our GSD puppy chewed it for the past 10 months and she is a pretty strong chewer. Only now did she manage to get a couple small pieces off of it, and WoofBite was quick to provide our one-time replacement. Great customer service! Will purchase more of their products and highly recommend!
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Reviewed in the United States on April 17, 2026
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Verified Purchase
T. M
San Leandro, US
★★★★★ 4
So far so good
Had this for almost a month and no chunks have been chewed off yet. I have a lab who prefers chewing over pulling or retrieving type toys. She's destroyed everything we've given her --sometimes within minutes. She loves Wubbas but she chews them to bits immediately. Other toys get chunks or small pieces chewed off and we wind up throwing them away. This has lasted well. Full disclosure, we only pull out toys at the end of the day for just a couple of hours, and we are within sight of her the whole time, that's why 4 stars. But so far to our surprise this has held up very well.
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Reviewed in the United States on August 9, 2025

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